Part:BBa_K5048067:Design

T7 promoter-HSP60-FLAG

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 51
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 92
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 51
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 51
  Illegal NgoMIV site found at 433
  Illegal AgeI site found at 588
  Illegal AgeI site found at 1066
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

HSP60 protein with a FLAG tag on the C-terminus and a T7 promoter to ensure the expression of the protein.Used to test the expression of HSP60.

Source

See our parts: K5048047, K5048060